Here we used Fluorescenceactivatedcell sorting

Posted by Ellie on Jul 28, 2008 in Comparative Transcriptomics | 0 comments

Studiesof gene expression in male gametes of maize and Plumbago, andin lily generative cells already showed that the previouslyheld view of transcriptionally inert male gametes was not true,but genomewide studies were lacking. Articles by Becker, J. These results provide roadmap to decipherthe role of spermexpressed proteins. In flowering plants the two sperm cells are embedded withinthe cytoplasm of the growing pollen tube and as such are passivelytransported to the embryo sac, wherein double fertilizationoccurs upon their release. Becker Instituto Gulbenkian de Ciencia Centro de Biologia do Desenvolvimento, Oeiras, 2780901, Portugal Plant Gene Expression Center, USDAARSUCBerkeley, Buchanan St., Albany, CA, 94710 Depto.

Comparative analysis of the transcriptome of sperm cellsat genomewide

Studiesof gene expression in male gametes of maize and Plumbago, andin lily generative cells already showed that the previouslyheld view of transcriptionally inert male gametes was not true,but genomewide studies were lacking. These results provide roadmap to decipherthe role of spermexpressed proteins..

Functional classifications of genes with enriched expressionin sperm cells showed that DNA repair, ubiquitinmediated proteolysisand cell cycle progression are overrepresented Gene Ontologycategories. Here we used Fluorescenceactivatedcell sorting FACS to isolate sperm cells from Arabidopsis,allowing GeneChip analysis of the transcriptome of sperm cellsat genomewide level. Articles by Becker, J. Analyses in the modelplant Arabidopsis thaliana were hindered because no method toisolate sperm cells was available.

Feijo , and Jorg Studiesof gene expression in male gametes of maize and Plumbago, andin lily generative cells already showed that the previouslyheld view of transcriptionally inert male gametes was not true,but genomewide studies were lacking. Functional classifications of genes with enriched expressionin sperm cells showed that DNA repair, ubiquitinmediated proteolysisand cell cycle progression are overrepresented Gene Ontologycategories. Articles by Becker, J.

Functional classifications of genes with enriched expressionin sperm cells showed that DNA repair, ubiquitinmediated proteolysisand cell cycle progression are overrepresented Gene Ontologycategories. Biologia Vegetal, Faculdade de Ciencias da Universidade de Lisboa Lisboa, Campo Grande, Portugal Corresponding author email jfeijoatfc. ul. pt Articles by Becker, J. PubMed PubMed Citation Articles by Borges, Moreover, analysis of the transcriptome of sperm cellsat genomewide level. Studiesof gene expression in male gametes of maize and Plumbago, andin lily generative cells already showed that the previouslyheld view of transcriptionally inert male gametes was not true,but genomewide studies were lacking.
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