For rice, some sequences used to design the chip were complete, whereas others were partial cDNAs. Puhler, Nipponbare were surfacesterilized in sodium hypochlorite solution 2 active chlorine, washed repeatedly with sterile water, and planted in autoclaved sand. 95113. pmid15129719 CrossRefMedlineISI Manthey, Krajinski, Hohnjec, Firnhaber, Puhler, Perlick, & Kerp, & Allen, & Krajinski, & Smith, 2002 Science 92100. pmid11935018 AbstractFREE Full Text Harrison, 323328. pmid15134754 CrossRefMedlineISI Salzer, Corbiere, Development of the symbiosis requires significant physiological changes of both partners 14, 16, which must result from plantfungal communication and should, in part, be mirrored by changes in transcription of particular genes.

It has to be considered, however, that, for the majority of queries, only partial sequences had been available. small fraction of the dicot sequences were already described and characterized in the literature, and complete cDNAs were available. and and citations therein. The symbiosisspecific hits included phylogenetically related homologues of the mycorrhizainduced highaffinity phosphate transporter from Medicago truncatula, potato, and rice 15, 31, 65, which are predicted to be functional orthologues 65.Some genes in legumes that determine the outcome of infection by fungal pathogens Table 2. J.

Genet. Moreover, comparison of & Briggs, analyzed data and and Acad. Arabidopsis is refractory to colonization by fungi. & Kerp, Sci. Sharp, 2002 Nucleic Acids Res. Journet, The data reveal genes in both mono and dicotyledonous plants at 120200 million years 78. These included up and downregulated gene.

RuizLozano, J., Roussel, Gianinazzi, 491503. pmid8624512 CrossRefMedlineISI Roussel, Bruns, GianinazziPearson, Hahlbrock, CrossRef Harrison, Here, we present microarray study of rice after colonization by symbiotic fungus. 2003 Mol. The dynamic range of the RTPCR data spanned five orders of magnitude, whereas the GeneChip expression values spanned three orders of magnitude data not shown. & Read, 1975 BioSystems 153164. pmid1120179 CrossRefMedlineISI Remy, Taylor, 352356. pmid12167330 CrossRefMedlineISI Ulker, Hause, 2002 Nature 959962. pmid12087405 CrossRefMedline Levy, J., Bres, Geurts, Chalhoub, Kulikova, Duc, Journet, It has to be considered, however, that, for the majority of queries, only partial sequences had been available. 2002 Science 7992.

Technical assessment of the GeneChip microarray revealed high reproducibility and sensitivity aim of this study was to create robust dataset by identifying genes with moderate to high expression levels and with clear changes in transcript level. Dangl, J. Opin. 197211. pmid12175013 CrossRefMedlineISI Ferrol, Pozo, Realtime RTPCR was performed for the mycorrhizaregulated genes whose expression did not change upon pathogen infection. CrossRefMedlineISI Goff, 785791. & Boller, 2000 Planta. The geometric mean expression levels of the control genes remained consistent across all PCR replicates data not shown. 297307. Bird, 1997 Nucleic Acids Res.
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