The overall aim of the course is to provide each student with the fundamental knowledge and handson experience necessary to be able to perform and analyze proteomics experiments, and to learn to identify new opportunities in applying proteomics approaches to hisher own research.. Students will learn to use several informatics tools available for analyzing the data.

The course will cover both MALDI and ESI highsensitivity mass spectrometry including peptide mass mapping and tandem mass spectrometry, quantification, and phosphoproteomics. In the functional proteomics section of the course, students will learn about cuttingedge protein separation methods, including handon experience with 2D gel electrophoresis, multidimensional liquid chromatography and affinity purification of protein complexes. Students will use robots to execute highthroughput methods including expression, purification and characterization of proteins.

Students will learn to use several informatics tools available for analyzing the data. Students will use robots to execute highthroughput methods including expression, purification and characterization of proteins. In the profiling section of the course, students will learn about cuttingedge protein separation methods, including handon experience with 2D gel electrophoresis, multidimensional liquid chromatography and affinity purification of protein complexes.

Speakers in the course included Ron Beavis, University of British Columbia Claudio Calonder, Zeptosens Brian Chait, The Rockefeller University Pierre Chaurand, Vanderbilt University School of Medicine This intensive laboratory and lecture course will focus on two major themes in proteomics protein profiling and functional proteomics. They will also print and analyze their own selfassembling protein microarrays, which will be used for proteinprotein interaction studies.

The overall aim of the course is to provide each student with the fundamental knowledge and handson experience necessary to be able to perform and analyze proteomics experiments, and to learn to identify new opportunities in applying proteomics approaches to hisher own research.. Students will use robots to execute highthroughput methods including expression, purification and characterization of proteins.