The high frequency with which we detected such

The comparative analysis suggests correlation between transcriptional and translational expression for the majority of genes. comparison of RNA and protein levels will be required. We used DNAchip based RNA expression profiling, 2Dgel electrophoresis and subsequent peptide mass fingerprinting to explore the general feasibility of such comparative gene expression analysis in mouse tissues. The understanding of this functional regulation is generally limited to distinct signalling or metabolic pathways.

comparison of RNA and protein levels will be required. We used DNAchip based RNA expression profiling, 2Dgel electrophoresis and subsequent peptide mass fingerprinting to explore the general feasibility of such comparative gene expression analysis in mouse tissues. Significant exceptions from this correlation confirm the complementarities of both approaches. We used this set of differential expression profiles as tool to address three major questions Does protein expression correlate with colocalisation in the genome? Whereas protein analyses mostly identify known metabolic enzymes and structural proteins, transcriptome analyses reveal the differential expression of functionally diverse and not yet described gene clusters.

The choice of these tissues provided large set of differentially expressed proteins and genes. Significant exceptions from this correlation confirm the complementarities of both approaches. biochemical processes within and between cells are put into effect by the interaction between proteins, or between proteins and their substrates Ge et al., 2001 Li et al., 2004 Walhout and Vidal, 2001 The comparative analysis suggests correlation between transcriptional and translational expression for the majority of genes. Thus, transcriptome and proteome feed back to each other in highly complex and controlled way.

Based on RNA expression data from the two hundred differentially expressed genes, we identify chromosomal colocalization of known as well as not yet described genes. The comparative analysis suggests correlation between transcriptional and translational expression for the majority of genes. The high frequency with which we detected such clusters suggest that coexpression of colocalizing genes is probably common.. biochemical processes within and between cells are put into effect by the interaction between proteins, or between proteins and their substrates Ge et al., 2001 Li et al.

Significant exceptions from this correlation confirm the complementarities of both approaches. Vice versa, the transcriptome can be regarded as sensitive readout of the proteome or the biochemical state of the cell. Thus, transcriptome and proteome feed back to each other in highly complex and controlled way. biochemical processes within and between cells are put into effect by the interaction between proteins, or between proteins and their substrates Ge et al., 2001 Li et al.