We found sperm express many genes, and that the proteins encoded by these genes encompass many different categories. We identified numerous candidate genes whose involvement in sperm cell development and fertilization can be directly tested in Arabidopsis. USA. gov. Pacific Plant Gene Expression Center Albany_CA Title Comparative Transcriptomics of Arabidopsis thaliana Sperm Cells Authors Borges, Filipe GULBENKIAN INST PORTUGAL Submitted to Plant Physiology Publication Type Peer Reviewed Journal Publication Acceptance Date 27, Publication Date 30, Publishers URL httpwww. plantphysiol.

This data can be used as starting point to discover the roles of these spermexpressed proteins. Project Team McCormick, Sheila Publications Publications Related National Programs Plant Biological and Molecular Processes 302 Plant Genetic Resources, Genomics and Genetic Improvement 301 Related Projects ELUCIDATING THE MECHANISMS THAT CONNECT ACTIVATION OF ROP GTPASES, MEMBRANE DYNAMICS AND SIGNALING IN CELL GROWTH AND STRESS RESPONSES Last Modified ARS Home.

Technical Abstract In flowering plants the two sperm cells are embedded within the cytoplasm of the growing pollen tube and as such are passively transported to the embryo sac, wherein double fertilization occurs upon their release. Interpretive Summary We purified sperm cells from Arabidopsis plants. Studies of gene expression in male gametes of maize and Plumbago, and in lily generative cells already showed that the previously held view of transcriptionally inert male gametes was not true, but genomewide studies were lacking.

Comparative analysis of the sperm cell transcriptome with those of representative sporophytic tissues and of pollen showed that sperm has distinct and diverse transcriptional profile. Comparative Transcriptomics of Arabidopsis thaliana Sperm Cells Authors Borges, Filipe GULBENKIAN INST PORTUGAL Submitted to Plant Physiology Publication Type Peer Reviewed Journal Publication Acceptance Date 27, Publication Date 30, Publishers URL httpwww. plantphysiol. org Reprint URL Citation Borges, Gomes, Gardner, Moreno, Mccormick, Feijo, J., Becker, J. Comparative Transcriptomics of Arabidopsis thaliana Sperm Cells Authors Borges, Filipe GULBENKIAN INST PORTUGAL Submitted to Plant Physiology Publication Type Peer Reviewed Journal Publication Acceptance Date 27, Publication Date 30, Publishers URL httpwww. plantphysiol.

Functional classifications of genes with enriched expression in sperm cells showed that DNA repair, ubiquitinmediated proteolysis and cell cycle progression are overrepresented Gene Ontology categories
Tags: citation, gene ontology, transcriptomics