About times more genes were significantly divergently expressed in ovary in comparison between XM and HXLXM than between XL and HXLXM. With goal of further exploring these results, we analyzed new expression data from single tissue from one species or one type of hybrid either HXLXB or HXLXM as treatment.Probemasks are lists of genes that are defined priori to be excluded from analysis before microarray normalization is performed.

Comparisons were made between testis and ovary expression profiles of testes or ovaries of XL and XM were compared to the same tissue in their hybrids, widespread dominance in expression was reported in hybrids wherein the expression profile of HXLXM tended to be more similar to XL than to the nontarget parental species XM One tactic is to select probes on the basis of genomic DNA gDNA hybridizations of the target and nontarget species to the microarray chip The resulting probemask included probes in total of probesets, for an average of probes per probeset.

For each species or hybrid in this study, three biological replicates different individuals were performed per tissue.

Each probe within probeset is an oligonucleotide base pairs in length that hybridizes to unique portion of an XL transcript. In this study, we analyzed data using two types of probemasks. Hereafter we refer to this probemask as the XBXL perfect match probemask. Recently, for example, the Xenopus laevis Affymetrix microarray chip was used to explore expression divergence between species . If the same amount of gDNA is used in the hybridization, probes that match conserved regions should hybridize with similar intensity to gDNA in both species .

For each species or hybrid in this study, three biological replicates different individuals were performed per tissue. Comparisons were made between testis and ovary expression profiles of testes or ovaries of XL and XM were compared to the same tissue in their hybrids, widespread dominance in expression was reported in hybrids wherein the expression profile of HXLXM tended to be more similar to XL than to the nontarget parental species XM Differences in technical procedures between laboratories and genetic differences among populations or individuals can also contribute to variation in expression divergence.
Tags: amp, nucleotide